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. 2020 May 28;18:56. doi: 10.1186/s12958-020-00596-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Sperm acetylome and phosphorylome analyses. (a) Acetylated sperm proteins (acetylated lysine) with major bands. (b) Densitometric analysis of the ratio of candidate bands. (c) Phosphorylated sperm proteins (phosphorylated tyrosine) with major bands. (d) Densitometric analysis of the ratio of 100- to 85-kDa bands. (d’) The ratio of 37–40-kDa (moderate) to 85–100-kDa (high) molecular weight bands. Differential counting was expressed as means (ranges) of three independent experiments. Differences were tested by two-way ANOVA, followed by Tukey’s multiple comparison test, and asterisks indicate statistical significance *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. (e) Candidate acetylated and (f) phosphorylated proteins from individual bands were evaluated using MALDI-TOF MS-based peptide detection. Analysed sperm samples represent a pool of three animals per experimental group from three independent replicates. (g) Densitometric analysis of acetylated tubulin from BPS1-treated sperm. VC: vehicle control, BPS1–3: increasing doses of bisphenol S