Fig. 5.

LINC00520 sponges miR-125b-5p in melanoma cells. a FISH showed that LINC00520 was mainly distributed in both cytoplasm and nucleus in melanoma cells. Scale bar, 25 μm. b qRT-PCR of nuclear and cytoplasm RNA fractions detected the LINC00520 expression in cytoplasm and nuclear. c The binding sites of miR-125b-5p on LINC00520, and target sequences were mutated. d Luciferase assay of melanoma cells transfected with LINC00520-WT or LINC00520-MUT reporter together with miR-125b-5p or NC. e MS2-RIP was used to detect the endogenous miR-125b-5p associated with the MS2-tagged LINC00520. f Melanoma cells transfected with biotin-labeled miR-125b-5p, mutated or NC oligos, and assayed by biotin-based pull down. The expression of LINC00520 were detected by qRT–PCR. g The levels of miR-125b-5p in melanoma cells following transfection with LINC00520 siRNA or NC. Data were expressed as the mean ± SD, **P < 0.01, ***P < 0.001