Fig. 7.

LINC00520 promotes the growth and metastasis of melanoma cell through miR-125b-5p/EIF5A2 axis. a Western blots identified EIF5A2, N-cadherin, E-cadherin and Vimentin protein expression changes in NC, miR-125b-5p mimic, miR-125b-5p mimic plus EIF5A2, si-LINC00520 or si-LINC00520 plus miR-125b-5p inhibitor transfected melanoma cells, GAPDH was used as a control. b Effect of miR-125b-5p mimic and si-LINC00520 on the proliferative ability of melanoma cells was determined by CCK8 assay. The results were further confirmed by co-transfection EIF5A2 plasmid and miR-125b-5p inhibitor respectively. c The DNA synthesis of melanoma cells grown was detected by EdU assay following transfection with NC, miR-125b-5p mimic, miR-125b-5p mimic plus EIF5A2, si-LINC00520 or si-LINC00520 plus miR-125b-5p inhibitor. Scale bar, 100 μm. d Effect of miR-125b-5p mimic and si-LINC00520 on the invasive capacity of melanoma cells was assessed by transwell assay. The results were verified by the recovery experiment of co-transfection EIF5A2 plasmid and miR-125b-5p inhibitor respectively. Scale bar, 50 μm. e Migration of melanoma cells was detected by scratch wound assay. EIF5A2 plasmid and miR-125b-5p inhibitor reversed the effect of miR-125b-5p mimic and si-LINC00520 on the migration capability of melanoma cells respectively. Scale bar, 100 μm. Data were expressed as the mean ± SD, *P < 0.05, **P < 0.01