Fig. 8.

LINC00520 exerts its pro-growth and pro-metastasis activity through regulating miR-125b-5p/EIF5A2 axis in vivo. a The schema of the animal experiment. b The excision tumor in nude mice of A375 cells that stably expressing LINC00520 shRNA or control xenografts. c Differences in the volume of tumor among groups. d The tumor weight of excised tumor tissues. e Xenograft tumour tissues were confirmed by H&E staining. Scale bar, 50 μm. f Expressions of LINC00520 and miR-125b-5p in xenograft tumour tissues were detect by qRT-PCR. g The expression of LINC00520 and EIF5A2 were examined by FISH and immunohistochemistry of sections from the xenograft tumour tissues. Scale bar, 25 μm. h Representative bioluminescence images of mice after tail vein injection of stably expressing LINC00520 shRNA or control A375 cells. i The excision lung tissues in nude mice, LINC00520 shRNA caused a decrease in the number of metastatic lung nodules. j Metastatic lung nodules were confirmed by H&E staining. Scale bar, 50 μm. k qRT-PCR detected the LINC00520 and miR-125b-5p level in the metastatic lung nodules. l The expression of LINC00520 and EIF5A2 were detected by FISH and immunohistochemistry of sections from the metastatic lung nodules. Scale bar, 25 μm. Data were expressed as the mean ± SD, *P < 0.05, **P < 0.01