Figure 4.
(a) Concentration-dependent inhibitory effect of D8 on NS3/4A protease activity used as a standard control. (b) Effect of D8 on cellular serine protease activity. Trypsin was incubated with the substrate in the presence of increasing concentrations of D8. Inhibitions were compared with a standard control reaction with no test compound (Std) as 100%. In this assay, the G. max soyabean inhibitor14 was used as a positive control. Symbol * indicates a statistically significant difference in terms of the P value (<0.05).