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Single-particle analysis of a simulated HEWL data set with PP. (a) A typical micrograph of HEWL with PP; the scale bar is 50 nm. (b) 2D class averages. (c) The 3D reconstruction of HEWL at 2.78 Å resolution from 225 751 particles. (d) Gold-standard Fourier shell correlation (FSC) before (blue line) and after (orange line) masking, and the phase randomized FSC (yellow line).
