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. 2020 Mar 4;29(8):1253–1273. doi: 10.1093/hmg/ddaa034

Figure 7.

Figure 7

DNM2 haploinsufficiency is not the likely underlying mechanism of the myopathy in Dnm2 K562E mice. (A) Exemplary cryosections of soleus muscles derived from 2-month-old control and Dnm2 wt/0 mice probed with antibodies targeting laminin or CD68. Exemplary CD68-positive macrophages (white arrowheads) are highlighted. Scale bar: 100 μm, refers to whole panel. (B) Quantification of CD68-positive macrophages in between myofibres reveals no significant changes between control and Dnm2 wt/0 muscles at 2 months. Bar heights, mean; error bars, SEM (n = 5 mice/genotype, images from two to three sections quantified and averaged per mouse). (C) (Left) Exemplary H&E-stained soleus muscle cryosections derived from control and Dnm2 wt/0 mice at 2 months. Scale bar: 100 μm, refers to whole panel. (C) (Right) Quantification of myofibre diameter frequency reveals no detectable difference between both genotypes on soleus muscle sections at 2 months. Datapoints, mean; error bars, SEM (n = 5 mice/genotype, images from three sections quantified and averaged per mouse). Two-tailed unpaired Student’s t-test (B) and two-way ANOVA with Sidak’s multiple comparisons test (C).