Figure 3.
Previously unresolved domain of Rg3GUS. (A) Structure of Rg3GUS (PDB 6MVG) with regorafenib-glucuronide docked at active site (spheres in green). The yellow portion has not been resolved in the crystal structure and is modeled from extant GUS structure BuGUS2 (5UJ6). (B) Progress curves of WT and STP641 mutant with 4-MUG showing decreased activity of mutant.