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. Author manuscript; available in PMC: 2020 May 28.
Published in final edited form as: Cell Rep. 2019 Dec 10;29(11):3421–3434.e8. doi: 10.1016/j.celrep.2019.11.018

Figure 1. Activation of TAZ is necessary and sufficient to promote brain metastases of lung adenocarcinoma cells.

Figure 1.

A) Representative images (day 30 post-injection) and B) analysis of brain metastasis-free survival (BMFS) in mice injected intracardially with PC9-pFuLT or pFuLT-Tet-TAZ4SA-expressing cells. Mice were given dox water for the duration of the study. Statistical analysis calculated by Log-rank (Mantel-Cox) test. Parental (n=13), TAZ4SA (n=17). C-D) Quantitative analysis (day 30 post-injection) of brain-metastatic index in mice injected intracardially with C) PC9-pFuLT (n=13) vs. PC9-pFuLT-Tet-TAZ4SA (n=17) or D) HCC4006-pFuLT (n=9) vs HCC4006-pFuLT-Tet-TAZ4SA (n=10) cells. Statistical analysis calculated by unpaired two-tailed t test. ** p-value < 0.01. E) Representative images of ex vivo mouse brains on day 32 post-injection with HCC4006-pFuLT or HCC4006-Tet-TAZ4SA cells. F) Diagram of iterative derivation of brain-metastatic cell lines. G) GSEA plot of TAZ4SA signature in PC9 parental vs PC9-BrM3 RNA-seq dataset. NES=normalized enrichment score. H) Venn diagram of overlapping transcripts upregulated in PC9-BrM3 and PC9-TAZ4SA cells. I) Representative images (day 42 post-injection) and J) BMFS in mice injected intracardially with PC9-BrM3 cells expressing non-target control (shNTC, n=10) or shTAZ (clone #73, n=10). K) Immunoblot to evaluate TAZ shRNA knockdown in PC9-BrM3 cells. Actin was used for protein loading control.