KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| AXL (C89E7) | Cell Signaling | 8661S; RRID:AB_11217435 |
| Phospho-AXL (Tyr702) (D12B2) | Cell Signaling | 5724S; RRID:AB_10544794 |
| Phospho-AXL (Tyr779) | R&D Systems | AF2228; RRID:AB_2062560 |
| ABL1 (8E9) | Millipore Sigma | MAB1130; RRID:AB_2220996 |
| ABL2 (6D5) | Abnova | H00000027-M03; RRID:AB_828506 |
| YAP/TAZ (D24E4) | Cell Signaling | 8418S; RRID:AB_10950494 |
| TAZ (V386) (immunoblotting) | Cell Signaling | 4883S; RRID:AB_1904158 |
| L1CAM (D5N9S) | Cell Signaling | 89861S; RRID:AB_2800145 |
| beta-Actin (8H10D10) | Cell Signaling | 3700S; RRID:AB_2242334 |
| Phospho-CrkL (Tyr207) | Cell Signaling | 3181L; RRID:AB_331068 |
| CRKL (C-20) | Santa Cruz | sc-319; RRID:AB_631320 |
| beta-Tubulin (D2N5G) | Cell Signaling | 15115S; RRID:AB_2798712 |
| FLAG (M2) | Millipore Sigma | F1804; RRID:AB_262044 |
| GFP | Roche Diagnostics | 11814460001; RRID:AB_390913 |
| GAPDH (6C5) | Santa Cruz | sc-32233; RRID:AB_627679 |
| Lamin B1 (D9V6H) | Cell Signaling | 13435S; RRID:AB_2737428 |
| TAZ (M2-616) (immunofluorescence) | BD Biosciences | 560235; RRID:AB_1645338 |
| TAZ (ChIP-qPCR) | Millipore Sigma | HPA007415; RRID:AB_1080602 |
| Alexa Fluor 568 Phalloidin | Thermo Fisher | A12380 |
| Phosphotyrosine (clone 4G10) | Millipore Sigma | 05-321; RRID:AB_309678 |
| Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson Immunoresearch | 115-035-003; RRID:AB_10015289 |
| Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | 115-035-144 |
| Normal Mouse IgG | Santa Cruz | sc-2025; RRID:AB_737182 |
| Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) | Cell Signaling | 58802S; RRID:AB_2799549 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant human GAS6 protein, CF | R&D Systems | 885-GSB-050 |
| GNF-5 (Abl kinase small molecule inhibitor) | Duke Small Molecule Synthesis Facility | N/A |
| ABL001 (Asciminib) | Duke Small Molecule Synthesis Facility | N/A |
| BGB324 (R428) | MedKoo Biosciences | 401810 |
| Doxycycline Hyclate ≥98% (for in vivo studies) | Acros Organics | 103516-794 |
| Doxycycline Hyclate (for in vitro studies) | Millipore Sigma | D9891 |
| Critical Commercial Assays | ||
| Cell Titer Glo | Promega | G7571 |
| Q5 Site-Directed Mutagenesis Kit | New England Biolabs | E0554S |
| Caspase-GLO 3/7 Assay | Promega | G8091 |
| Corning BioCoat Matrigel Invasion Chambers | Corning | 354480 |
| Deposited Data | ||
| PC9 Parental versus PC9-TAZ4SA RNA-seq | This paper | GEO: GSE130425 |
| PC9 Parental versus PC9-BrM3 RNA-seq | (Boire et al, 2017) | GEO: GSE83132 |
| Experimental Models: Cell Lines | ||
| PC9 | (Valiente et al, 2014) | N/A |
| PC9-BrM3 | This paper | N/A |
| HCC4006 | ATCC | CRL-2871; RRID:CVCL_1269 |
| HCC4006-BrM | This paper | N/A |
| NCI-H1975 | ATCC | CRL-5908; RRID:CVCL_1511 |
| H1975-BrM3 | This paper | N/A |
| HCC827 | ATCC | CRL-2868; RRID:CVC:_2063 |
| Experimental Models: Organisms/Strains | ||
| Mouse outbred athymic nu/nu | Jackson Laboratory | 007850; RRID:IMSR_JAX:007850 |
| Oligonucleotides | ||
| See Table S2 for a list of all primers and oligonucleotides. | ||
| Recombinant DNA & Plasmids | ||
| pLKO-puro Non-Target shRNA Control | Sigma Mission TRC1 | SHC016-1EA |
| pLKO-puro shAXL 1040 | Sigma Mission TRC1 | TRCN0000001040 |
| pLKO-puro shTAZ 70 | Sigma Mission TRC1 | TRCN0000019470 |
| pLKO-puro shTAZ 71 | Sigma Mission TRC1 | TRCN0000019471 |
| pLKO-puro shTAZ 73 | Sigma Mission TRC1 | TRCN0000019473 |
| TetO-FUW-pgk-puro | (Chowdhury et al., 2016) | Addgene #85747; RRID:Addgene_85747 |
| pLVX-Tight-Puro Tet-On Vector | Xaralabos Varelas, Boston University, Boston, MA, USA | N/A |
| pLVX-TP-3F-TAZ4SA | Xaralabos Varelas, Boston University, Boston, MA, USA | N/A |
| Scramble control shRNA | (Gu et al, 2016) | N/A |
| ABL1 shRNA | (Gu et al, 2016) | N/A |
| ABL2 shRNA | (Gu et al, 2016) | N/A |
| 3XFlag pCMV5-TOPO TAZ wild type | (Varelas et al., 2008) | Addgene #24809; RRID:Addgene_24809 |
| 3XFlag pCMV5-TOPO TAZ Y321F | This paper | N/A |
| Tet-pLKO-puro | (Wiederschain et al., 2009) | Addgene #21915; RRID:Addgene_21915 |
| Tet-pLKO-shNTC-puro | This paper | N/A |
| Tet-pLKO-shAXL-puro | This paper | N/A, shAXL clone TRCN0000001040 |
| Tet-pLKO-shTAZ-puro | This paper | N/A, shTAZ clone TRCN0000019470 |
| N174-MCS | http://n2t.net/addgene:81061 | Addgene #81061; RRID:Addgene_81061 |
| phL1A-pcDNA3 | (Hlavin and Lemmon, 1991) | Addgene #12307; RRID:Addgene_12307 |
| N174-L1CAM | This paper | N/A |
| pN1-eGFP | Anthony Koleske, Yale University, New Haven, CT, USA | N/A |
| pN1-ABL2-eGFP WT | Anthony Koleske, Yale University, New Haven, CT, USA | N/A |
| pN1-ABL2-eGFP R198K | This paper | N/A |
| pN1-ABL2-eGFP K317M | This paper | N/A |
| pWZL Neo Myr Flag AXL | (Boehm et al., 2007) | Addgene #20428; RRID:Addgene_20428 |
| pWZL Neo Myr Flag AXL Y779F | This paper | N/A |
| pWZL Neo Myr Flag AXL Y821F | This paper | N/A |
| pWZL Neo Myr Flag AXL Y830F | This paper | N/A |
| pWZL Neo Myr Flag AXL Y866F | This paper | N/A |
| Software and Algorithms | ||
| Prism 6 and 8 | Graphpad | http://graphpad.com/scientific-software/prism |
| ImageJ | (Schneider et al., 2012) | http://imagej.nih.gov |
| RStudio | R Foundation for Statistical Computing | http://rstudio.com |
| CBLigand Online BBB Predictor Tool v0.90 | (Liu et al., 2014) | http://cbligand.org |
| Living Image | Perkin Elmer | http://perkinelmer.com |
| GSEA 3.0 | (Subramanian et al., 2005) | http://software.broadinstitute.org/gsea |
| KM Plotter | (Gyorffy et al., 2013) | http://kmplot.com |
| CBioPortal v3.1.1 | (Cerami et al., 2012; Gao et al., 2013) | http://cbioportal.org |
| TrimGalore v0.4.4 | Babraham Bioinformatics | http://github.com/FelixKrueger/TrimGalore |
| STAR aligner | (Dobin et al., 2013) | http://github.com/alexdobin/STAR |
| HTSeq-count | (Anders et al., 2015) | http://htseq.readthedocs.io |
| DESeq2 v2.12 | (Love et al., 2014) | http://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Hisat2 v2.1.0 | (Kim et al., 2019) | http://ccb.jhu.edu/software/hisat2/index.shtml |