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. 2020 Apr 29;44(1):139–155. doi: 10.3892/or.2020.7601

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — miR-193b inhibits proliferation via KRAS and suppresses migration and invasion via STMN1. (A) Transfection efficiency was verified after F4 and F5M2 cells were transfected with siKRAS or pMSCV-KRAS, respectively. (B) Transfection efficiency was verified after F4 and F5M2 cells were transfected with siSTMN1 or pMSCV-STMN1, respectively. (C) Co-transfection with pMSCV-KRAS reversed miR-193b-mimic induced cell cycle arrest at G₁ phase in F5M2 cells, whereas co-transfection with siKRAS reversed the miR-193b inhibitor-induced increase in percentage of cells at G₂ phase in F4 cells. (D) Transwell cell invasion assay (magnification ×40). (E) wound-healing assay (magnification ×20) revealed that co-transfection of F5M2 cells with pMSCV-STMN1 and miR-193b mimic or co-transfection of F4 cells with siSTMN1 and miR-193b inhibitor reversed the regulatory effects of miR-193b mimic or inhibitor. Data are presented as the mean ± SD of at least three independent experiments. *P<0.05. miR-193b, microRNA-193b; NC, negative control; STMN1, stathmin 1; si, small interfering RNA.

Figure 5. — miR-193b inhibits proliferation via KRAS and suppresses migration and invasion via STMN1. (A) Transfection efficiency was verified after F4 and F5M2 cells were transfected with siKRAS or pMSCV-KRAS, respectively. (B) Transfection efficiency was verified after F4 and F5M2 cells were transfected with siSTMN1 or pMSCV-STMN1, respectively. (C) Co-transfection with pMSCV-KRAS reversed miR-193b-mimic induced cell cycle arrest at G₁ phase in F5M2 cells, whereas co-transfection with siKRAS reversed the miR-193b inhibitor-induced increase in percentage of cells at G₂ phase in F4 cells. (D) Transwell cell invasion assay (magnification ×40). (E) wound-healing assay (magnification ×20) revealed that co-transfection of F5M2 cells with pMSCV-STMN1 and miR-193b mimic or co-transfection of F4 cells with siSTMN1 and miR-193b inhibitor reversed the regulatory effects of miR-193b mimic or inhibitor. Data are presented as the mean ± SD of at least three independent experiments. *P<0.05. miR-193b, microRNA-193b; NC, negative control; STMN1, stathmin 1; si, small interfering RNA.