Figure 5.

D-JHU29 decreased the abnormal elevated glutamate release in brain slices from Mecp2-KO mice. A. Coronal slices (300 μm thick) containing the hippocampi were cut from fresh brain tissue of both WT and Mecp2-KO mice; each slice was plated individually. Twenty-four hours after plating, the culture media was changed to media alone, media +JHU29 or media + D-JHU29. Media was collected 24 hours later and analyzed for glutamate concentration. B. Mecp2-KO hippocampal slice cultures from 5-6 week old mice show increased glutamate production in tissue culture. D-JHU29 and free JHU29 (both at 100 μg/ml JHU29) decreased extracellular glutamate levels in Mecp2-KO slice culture. * p < 0.05, ** p < 0.01