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. 2020 Apr 27;10(13):5914–5931. doi: 10.7150/thno.43156

Figure 1.

Figure 1

Exosomal vesicles mediate interaction of HERS and DPCs cells (A) Schematic diagram showing transwell coculture of HERS-H1 and DPCs cells. (B) HERS-H1 cells were labeled by DiO (B i), DPCs cells were stained with phallotoxins, and nuclei were stained with DAPI (B ii). DPCs cells cocultured with HERS-H1 cells endocytosed exosomal vesicles (white arrow) released from HERS-H1 cells (B iii), whereas DPCs cells cocultured with GW4869-pretreated HERS-H1 cells did not show the endocytosed exosomal vesicles (B iv). (C) HERS-H1 cells upregulated the expression of DSPP and DMP1 in DPCs cells, which was attenuated by pretreatment with GW4869. (D) TEM analysis of ELVs. (E) DLS showed the particle size distribution of ELVs-H1. (F) Western blot analysis of the surface markers of ELVs. (G) DPCs cells were incubated with DiO-labeled ELVs (green) for 2, 24, and 48 h, respectively. Nuclei of DPCs cells were stained with DAPI (blue). (TEM: transmission electron microscope; DLS: dynamic light scattering). Scale bars are shown. *p < 0.05 vs. Con; #p < 0.05 vs HERS-H1.