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. 2020 Apr 27;10(13):5763–5777. doi: 10.7150/thno.38087

Figure 4.

Figure 4

Transcriptional upregulation of LC3B by JMJD2B during glucose deprivation. A. JMJD2B knockdown in HCT116 cells caused coordinate downregulation of autophagy lysosome genes. Values were taken from an Affymetrix gene microarray in triplicate experiments; P < 0.05 for each gene. B. Real-time PCR validation of the mRNA levels of LC3B in HCT116 and SW480 cells. Data are normalized to the expression level of ACTB (*P < 0.05, **P < 0.01, ***P < 0.001). C. Quantitative ChIP PCR analysis showing JMJD2B binding to the LC3B promoter in HCT116 and SW480 cells with JMJD2B knockdown and in control cells (*P < 0.05). D. Quantitative ChIP PCR analysis of the levels of H3K9 tri-methylation binding to the LC3B promoter in HCT116 and SW480 cells. Data are displayed as the fold change calculated relative to the controls (*P < 0.05, **P < 0.01, ***P < 0.001).