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. 2020 Apr 27;10(13):5704–5718. doi: 10.7150/thno.42494

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Fig 4 — Immunogenicity of the purified fusion proteins. (A) Immunization procedure. Immunization (white triangles) and bleeding time points are shown (red drops). (B) Anti-gp350 titers determination by ELISA. gp350ECD123 was immobilized on 96-well plates and the diluted sera were added for titers determination. The OD450=0.1 was set as the cut-off value. Significance (*p≤0.05, **p≤0.01, ***p≤0.001) between 149-3A and 149-3B VLPs versus gp350ECD123 is shown. Both constructs 149-3A and 149-3B have the same level of significance above gp350ECD123. (C) Binding titers of serum antibodies to the vector (HBc149) collected after eight weeks. The error bars indicated the standard deviation in each group (n=5 mice).