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. 2020 Apr 27;10(13):5719–5735. doi: 10.7150/thno.42010

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PTBP1 inhibits AS of Axl exon 10 by competitively inhibiting U2AF2. The U2AF2 binding sequence on Axl pre-mRNA was detected by CLRIP (A) and RNA pulldown assay (B). (C) Graphical representation of the MS2-GFP-IP system and its mode of operation. (D) The effect of PTBP1 over-expression or knockdown on the ability of U2AF2 to bind Axl pre-mRNA was investigated by the MS2-GFP-IP system. (E) The effect of PTBP1 on the ability of snRNAs to bind to Axl pre-mRNA was studied using the MS2-GFP-RIP system. The control levels were set to a value of 1. Data are presented as mean ± S.D. (N=3), two-way ANOVA followed by Tukey's test was performed for statistical analysis. The “*, **, ***” indicate “P<0.05, 0.01, 0.001” versus the control group, respectively.