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. 2020 Apr 27;10(13):5829–5844. doi: 10.7150/thno.44051

Figure 1.

Figure 1

Trehalose suppresses cisplatin-induced injury in vitro. Mannitol was used as osmotic control of trehalose. (A) HK2 cells were exposed to cisplatin (5 μM, 10 μM, 20 μM) for 24 h or 48 h, and cell viability was determined by CCK8. (B) HK2 cells treated with cisplatin were incubated with trehalose for 48 h, and cell viability was determined. (C) The effects of trehalose on cisplatin-induced apoptosis were determined by flow cytometry. (D) The expression of apoptosis-related proteins (Bax and Bcl-2 was measured by western blotting. B - D, cisplatin, 5 μM; trehalose, 50 mM; mannitol, 50 mM. Data are shown as the means ± s.d. from three independent experiments and analyzed by one-way ANOVA with Tukey's test. * P < 0.05, ** P < 0.01 vs. Con; & P < 0.05, && P < 0.01 vs. Cisp. (Con, control; Tre, trehalose; Cisp, cisplatin; Cisp + Tre, cisplatin + trehalose; Man, mannitol; Cisp + Man, cisplatin + mannitol).