Figure 10.

Oral treatment with trehalose alleviates cisplatin-induced AKI. (A) Mice were intraperitoneally injected with cisplatin, and trehalose was orally administered on the 2nd day after cisplatin injection. (B) Serum BUN and Crea levels were quantified in each group (n = 8 - 11 mice). (C) Representative images of HE- and PAS-stained kidney sections. (D - F) The expression of apoptosis-related proteins (Bax and Bcl-2), autophagy-related proteins (TFEB and LC3), and mitochondria-related proteins (Drp1, Opa1, ATP5a, Sirt3 and Ndufs4) was measured by western blotting (n = 3 mice). Data analyzed by one-way ANOVA with Tukey's test. * P < 0.05, ** P < 0.01 vs. Con; & P < 0.05, && P < 0.01 vs. Cisp. (NC, normal control; Cisp, cisplatin; Tre, trehalose; C + T, cisplatin + trehalose; i.g., intragastric; BUN, blood urea nitrogen; Crea, serum creatinine; HE, hematoxylin/eosin; PAS, periodic acid-Schiff).