TABLE 2.
Primers used in this study.
Primers | Sequence (5’–3’) | Source or reference |
For mutant construction | ||
wecA-up-F/R | CTGTCGACAACAATGACCATACCGAGTAATAAGC TTTGTGGTGTCGTTCGCCAGTCTGAGTCGTATGAA ACGCAGAGCGAAAAG | This study |
wecA-down-F/R | CTTTTCGCTCTGCGTTTCATACGACTCAGACTGGC GAACGACACCACAAA ATGCGGCCGCCCTAATGCGACACGGAAACC | This study |
wecA-extrrior-F/R | ACCGATTTCCGGCGCAAGAG TGGCGAAGCAAAGAACAGTAATAGAG | This study |
wecA-interior-F/R | TGCCCTTGACCTTGGGTGCTA TCTTGCGGCGGTGACTATTCTA | This study |
For complement construction | ||
wecA-F/R | GCGTCGACATGTGGCTTACTTTTCTTGCTGTTT TAGCGGCCGCTTACTCACTACCGATTTTGAGCTTTCTTTTC | This study |
For qRT-PCR | ||
16S rRNA-F/R | CCATGCCGCGTGAATGA TTCCTCGCTACCGAAAGAACTT | Subashchandrabose et al., 2013 |
WecA-F/R | ATACGCCGATGGTAGAGCAA ATCGATATGGTGGCGGTCAT | This study |
For emsa assay | ||
WecA-E-F/R | CTGAATAGCGTTGATCGACAAGGTT | This study |
GGGGTTAGCTCCCTCAAAATCAATA |