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. 2020 May 16;10(14):6411–6429. doi: 10.7150/thno.42602

Figure 4.

Figure 4

Modulation of MYC member expression upon glutamine deprivation and irradiation. (A) Representative western blot of MycN and c-Myc levels in BE(2)-C cells following MYCN downregulation upon glutamine deprivation and irradiation (4 Gy). GAPDH was used as a loading control. (B, C) Relative (B) MycN and (C) c-Myc protein expression analysed by densitometry in BE(2)-C cells following MYCN downregulation upon glutamine deprivation and irradiation (4 Gy) (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001). (D, E) Radiobiological colony forming assay of BE(2)-C cells following MYCN and c-MYC downregulation (D) with glutamine or (E) upon 24 h glutamine deprivation (n ≥ 3; ± S.E.M; ns p > 0.05). (F, G) Radiobiological colony forming assay of SH-SY5Y cells following MYCN and c-MYC downregulation (F) with glutamine or (G) upon 24 h glutamine deprivation (n ≥ 3; ± S.E.M; ns p > 0.05).