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. 2020 May 15;10(14):6182–6200. doi: 10.7150/thno.46553

Figure 1.

Figure 1

The injury of smooth muscle caused by specific knockout of Kindlin-2 leads to embryonic lethality. (A) Gross appearance of E12.5-15.5 embryos with different genotype. Embryos were fixed with paraformaldehyde. cKO (Kindlin-2fl/fl;SM22α-cre+) embryos are lethal at E14.5 (arrows). (B) Whole embryo sections were stained with hematoxylin and eosin (HE). Representative images and morphological abnormality are presented. The WT (Kindlin-2fl/fl;SM22α-cre-, Up panel) and cKO mice (Kindlin-2fl/fl;SM22α-cre+,Lower panel) were presented. H: heart; L: liver; GI: gastrointestinal tract; P: pancreas. Scale bar, 1mm. (C) HE staining of embryonic arteries from E12.5-E15.5. Scale bar, 50μm (D) Western blot analysis of whole embryo lysis from E12.4-E15.5, test for Myh11, α-SMA, CNN expression levels respectively. (E) Quantitation of (D) and two other independent experiments. Data were presented as the mean ± SEM. *p < 0.05 by student's t-test. See Figure S1, S2, Table S1, S2 and S3 for additional information.