Figure 4.

Depletion of Kindlin-2 in adult smooth muscle leads to apoptosis. (A) Kindlin-2, α-SMA, cleaved caspase-3 immunohistochemical staining of aortic cross-sections (left) and colon (right). WT and iKO mice were treated with tamoxifen injection parallelly, ascending aortas and sigmoid colons were excised and fixed 14 days after injection. Scale bar, 100μm. (B) Immunofluorescence of TUNEL (red), DAPI (blue) in combination with α-SMA (green) on cross-sections of ascending aortas (up) and sigmoid colons (below). Higher magnified views of highlighted regions were shown on the right. Scale bar, 20µm. See Figure S5 for additional information.