Skip to main content
. 2020 May 16;10(14):6483–6499. doi: 10.7150/thno.44025

Figure 4.

Figure 4

STIM1 replenish abrogates the anoikis resistance and metastasis of Snail1 OE-cells. (A) Effects of STIM1 on the proliferation of Snail1 OE-SMCC7721 and HepG2 cells. (B) Transwell assays for the invasion of WT-, Snail1 OE-, Snail1 plus STIM1 double OE (Snail1+STIM1 dOE)-SMMC7721 and HepG2 cells. (C, D) FACS (C) and caspase 3 activity assay (D) were used to measure the anoikis rate in mock-, Snail1 OE- and Snail1+STIM1 dOE-SMMC7721 and HepG2 cells. (E) The effects of STIM1 restoration on the metastasis of Snail1 OE-SMMC7721 and HepG2 cells in vivo. Lungs were observed for metastatic nodules on the surface, stained by H&E for histological analyses, arrows point to metastatic nodules. Representative photographs and H&E staining were shown (n = 4 mice per group). (F) Transwell assays were performed to detect the effects of different concentrations SKF-96365 on the invasion ability of Snail1 OE-SMMC7721 and HepG2 cells. (G) CCK-8 assay was applied to examine the effects of SKF-96365 with different concentrations on the survival of Snail1 OE-SMMC7721 and HepG2 cells. Data of (A-D, F and G) are expressed as mean ± SEM (n = 3). **p < 0.01, ***p < 0.001, ***p < 0.001, NS represents no significant difference.