Skip to main content
. 2020 May 16;10(14):6483–6499. doi: 10.7150/thno.44025

Figure 6.

Figure 6

Lacking of STIM1 rewires aerobic glycolysis towards AMPK-activated FAO. (A) PCR-array was applied to examine the expression changes of key metabolic genes caused by STIM1 KO and STIM1 KO+Snail1 OE (GSE148129). (B) Protein levels of indicated metabolic molecules in WT- and STIM1 KO-SMMC7721 cells. (C) Protein levels of LKB1/AMPK pathway in WT- and STIM1 KO-SMMC7721 cells. (D) The AMP/ATP ratio in WT- and STIM1 KO-SMMC7721 with or without glucose (20 mM). (E) Effects of glucose on the expressions of p-LKB (Ser428) and p-AMPK (Thr172) in STIM1 KO-SMMC7721 cells. (F) FAO in STIM1 KO-SMMC7721 cells transfected with si-NC or si-AMPKα. (G) Effects of ETO (100 μM) on the anoikis of STIM1 KO-SMMC7721 and HepG2 cells were examined by FACS, as well as their corresponding WT-group. ETO: etomoxir. Data are expressed as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.