Figure 2.

Knock-down of CDK12 induces cell cycle arrest and delays tumor progression in vivo. A. Cell cycle was detected by flow cytometry after CDK12 knock-down in SNU-1 and KATOIII cells 24 h after seeding the cells. B. Longitudinal tumor volume, tumor weight, final tumor size and percentage of mice with tumors less than 1000 mm³ at the endpoint were measured after the inoculation of shMock, shCDK12#2 and shCDK12#5 SNU-1 cells into NU/NU mice. Tumor size was measured every 3-7 days, and the volume was calculated by V = (length) × (width) × (height). The tumors in each group are shown. C. Longitudinal tumor volume and tumor weight were measured after injecting lentivirus (shMock, shCDK12#2 and shCDK12#5) directly into patient-derived xenografts (PDX) tumors at ~ 200 mm³. Two different cases (upper panels: LSG50, lower panels: LSG45) were tested. Tumor weight was measured at the endpoints. The tumors in each case are shown. Data represent means ±SD. P<0.05: *, p<0.01: **, p<0.001: ***. Significance determined by two-tailed Student's t test.