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. 2020 May 15;10(14):6322–6336. doi: 10.7150/thno.42581

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MOI interactome obtained by Cluster-FLIM. (A) Scheme and assessment of individual uptake steps of MOI 2 (NC-ICC) by amount and appearance of the FLIM clusters within the cell (cell membrane (M), cytoplasm (CP)). Nuclei are stained by DAPI (blue), plasma membranes by CellMask (dark red). Cyan, yellow, and red indicate the different FLIM clusters. (B) Temporal development of NC-ICC appearance. Values are mean ± SD. Fits according to the uptake and transport model (Eq. 8-12) are shown. Fit time constants: τ₁ = 8 ± 2 min (membrane receptor → caveolae), τ_-1 = 28 ± 11 min (back reaction of τ₁ ), τ₂ = 88 ± 6 min (internalization of receptor-NC-ICC complex by caveolae), τ₃ = 107 ± 9 min (transport to lysosome).