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. 2020 May 21;10:820. doi: 10.3389/fonc.2020.00820

Figure 4.

Figure 4

(A) mRNA evaluation of PGC1α in U937 cells treated with MC2494 at 50 μM concentration for 3 and 24 h. U937 cells were treated with MC2494 for the indicated times at 50 μM concentration. GAPDH was used as control for equal loading. Graphs show the mean of at least 2 independent experiments with error bars indicating standard deviation. (B) Top panel, western blot analysis of PGC1α; bottom panel western blot analysis of PGC1β. U937 cells were treated with MC2494 at 50 μM concentration for indicated times. Numbers on Western blot indicate the results of densitometry analysis, performed using the Image J Gel Analysis tool. (C) Immunofluorescence for PGC1α. Bar, 10 μM. Immunofluorescence microscopy analysis (right graph) is representative of 3 independent experiments. Values are mean ± standard deviation (SD) of biological triplicates. ***p ≤ 0.001, **p ≤ 0.01 vs. control cells.