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. 2020 May 21;11:490. doi: 10.3389/fphys.2020.00490

FIGURE 8.

FIGURE 8

Changes in Na+ transport across female rectal pad epithelia in response to saline (vehicle control), Droso-leucokinin and AedaeCAPA-PK1. Experiments were run only using hindgut preparations that were initially exhibiting hemolymph-directed ion flux (absorption) when unstimulated. Mean ± SEM obtained from 10 to 14 tissue preparations. There was a significant reduction in Na+ absorption in response to Droso-leucokinin (p < 0.05); however, ion transport did not significantly change following AedaeCAPA-PK1 treatment in comparison to basal transport activity (A), determined by a one-way ANOVA and Tukey’s post hoc test. Still images of the dissected rectum are demonstrated with ion flux recordings obtained from a sample measurement during baseline activity in saline (B,D) followed by either Droso-leucokinin (C) or AedaeCAPA-PK1 (E) application. Arrows indicate the direction and approximate the magnitude of Na+ transport, but are not drawn to scale. Scale bars, 100 μm.