Table 1.
Test results for determining the limit of detection of the LightMix® Modular SARS and Wuhan CoV E-gene assay with genomic RNA extracted from a SARS-CoV-2 culture isolate.
| Virus titer (TCID50/mL) | Cp (Intra-run) |
Cp (Inter-run) |
||||
|---|---|---|---|---|---|---|
| Test 1 | Test 2 | Test 3 | Test 1 | Test 2 | Test 3 | |
| 1.8 × 101 | 30.04 | 30.05 | 29.91 | 30.23 | 30.42 | 30.44 |
| 1.8 × 10° | 33.24 | 33.61 | 33.64 | 33.57 | 34.01 | 33.77 |
| 1.8 × 10−1 | 36.50 | 36.03 | 36.64 | 37.25 | 37.61 | 37.05 |
| 1.8 × 10−2 | 40.00 | 40.00 | – | – | – | 38.16 |
| 1.8 × 10−3 | 37.87 | – | – | – | – | – |
Abbreviations: -, negative; Cp, cycle number at detection threshold.