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Imprecision of the LightMix® Modular SARS and Wuhan CoV E-gene assay using the SARS-CoV-2 culture isolate extracts.
| Intra-assay | Inter-assay | ||
|---|---|---|---|
| Virus titer (TCID50/mL) | Number of positive replicates | Mean Cp ± SD (% coefficient of variation) |
Mean Cp ± SD (% coefficient of variation) |
| 1.8 × 101 | 3 | 30.00 ± 0.08 (0.26) | 30.18 ± 0.22 (0.72) |
| 1.8 × 10° | 3 | 33.50 ± 0.22 (0.67) | 33.64 ± 0.25 (0.75) |
| 1.8 × 10−1 | 3 | 36.39 ± 0.32 (0.88) | 36.85 ± 0.57 (1.54) |
