Table 3.

Primer combinations used for screening of porcine sapoviruses.

Primer Name	Sequence (5' to 3')	Function*	Location in genome	Strain	Accession number	Reference
p290**	GAT TAC TCC AAG TGG GAC TCC AC	Forward	4327–4349	GIII/Cowden	AF182760	Jiang et al., 1999.
p110**	DAC DAT YTC ATC ATC ACC ATA	Reverse	4674-4654			Le Guyader et al., 1996.
p290**	GAT TAC TCC AAG TGG GAC TCC AC	Forward	4327-4349	GIII/Cowden	AF182760	Jiang et al., 1999
p289**	TGA CAA TGT AAT CAT CAC CAT A	Reverse	4657-4636
p290h**	GAT TAC TCC AGG TGG GAC TCC AC	Forward	4327-4349	GIII/Cowden	AF182760	Farkas et al., 2004.
p290i**	GAT TAC TCC AGG TGG GAC TCA AC	Forward	4327-4349
p290j**	GAT TAC TCC AGG TGG GAT TCA AC	Forward	4327-4349
p290k**	GAT TAC TCC AGG TGG GAT TCC AC	Forward	4327-4349
p289h**	TGA CGA TTT CAT CAT CAC CAT A	Reverse	4657-4636
p289i**	TGA CGA TTT CAT CAT CCC CGT A	Reverse	4657-4636
SR80	TGG GAT TCT ACA CAA AAC CC	Forward	4339-4358	GIII/Cowden	AF182760	Vinjé et al., 2000.
JV33	GTG TAN ATG CAR TCA TCA CC	Reverse	4658-4639
PEC46	GTG CTC TAT TGC CTG GAC TA	Forward	4312–4331	GIII/Cowden	AF182760	Guo et al., 2001
PEC45	TCT GTG GTG CGG TTA GCC TT	Reverse	4883–4864
PEC66	GAC TAC AGC AAG TGG GAT TCC	Forward	4327–4347	GIII/Cowden	AF182760	Guo et al., 2001
PEC65	ATA CAC ACA ATC ATC CCC GTA	Reverse	4656–4636
nF	CTC GTA TGC TGA GGA CAC AC	Forward	4392–4411	GIII/Cowden	AF182760	Kim et al., 2006.
nR	GAG TGT CTG TTG GCT CAA TG	Reverse	4771 − 4752
CapsidF	GTG ATC AAC CCT TTT GAA AC	first, forward	5698–5717	GIII/Cowden	AF182760	Kim et al., 2006
PECVcapsidF	CTC GTC ATA GTA GGT GTG GC	second, forward	5890–5909
CapsidR/PECV capsidR	AAA GCA TGA TGT TGT TAG GC	first and second reverse	6454-6435
SaV1	GAT TAC TCC AGG TGG GAY TCM AC	Forward	4327-4349	GIII/Cowden	AF182760	Shen et al., 2009
SaV2	TGA CGA TTT CAT CAT CMC CRT A	Reverse	4657-4636
SaVR1	TGA CAA TGT AAT CAT CAC CAT A	Reverse	4657-4636
SaVR2	TGA CGA TTT CAT CAT CAC CAT A	Reverse	4657-4636
SaVR3	TGA CGA TTT CAT CAT CCC CGT A	Reverse	4657-4636
No name	GAT TAC TCC AGT GGA YTC MAC	Forward	4327-4349	GIII/Cowden	AF182760	Song et al., 2011
No name	TGA CGA TTT CAT CAT CMC CRT A	Reverse	4657-4636
SaVFp	ACA CCT ACT GGG TGA TGA TTG TGT G	Forward	4629-4653	GIII/Cowden	AF182760	Liu et al., 2012a; Liu et al., 2012b.
SaVRp	TGA GTG CCC TCT GGG TTG CTC G	Reverse	5192-5171
No name	GAA GAT GAA GAG CCA GAA GT	Forward	5113-5132	GIII/Cowden	AF182760	Zhang et al. 2014
No name	CCA TCG AGT TTC TCC ACC	Reverse	5641-5624
PSaV-F	TAC AGC AAG TGG GAC	Forward	4330–4344	GIII/Cowden	AF182760	Ding et al., 2019
PSaV-R	ATG ACA CTG GTG AAC GGC AT	Reverse	4526-4507
SaV-F	TAC GGG GGA ATA GGT TT	Forward	5855-5871	GIII/Cowden	AF182760	Jiang et al., 2019
SaV-R	CAG CCA CAT CTG GGT AGT	Reverse	6100-6083
PEC68	CCG CTA TAA ATT TAT TGG GTG	Forward	4260-4280	GVI/OH-JJ674	KJ508818	Wang et al., 2006
PEC67	ACG GGA CCC CAT ATT TTT GG	Reverse	4484-4465
SaV XF***	ATA TGA TGA GGG CTT TTG GCA T	Forward	4587-4608	GVI/OH-JJ674	KJ508818	Sisay et al., 2013
SaV XR***	CCC CTC CAT GAC ATA CAC TAC TG	Reverse	5011-4989
PSV11	CAC CCA GAG GTG ATT TCA ACA GCA	Forward	4207-4230	GVII/RV0042	KX000384	Wang et al., 2006
PSV14	TTC TGC GTA ACA CTG GAG CAC ACA	Reverse	4437–4414
PSV11M	CAC CCR GAG GGG ATC WCA	Forward	4207-4224	GVII/RV0042	KX000384	Sisay et al., 2013
PSV14M	TAA CAV TSV AGC ACA CAA CAT G	Reverse	4430-4409

*Primers used for semi-nested RT-PCR are indicated as first and second.

**These primers are universal primers for calicivirus, but not PoSaV-specific. So, their RT-PCR products should be sequenced for confirmation.

***These primers Also detected porcine kobuvirus.