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. 2020 May 18;11(15):4442–4452. doi: 10.7150/jca.42610

Figure 4.

Figure 4

Co-expression of PKM2 and PD-L1 with CD3, CD68 detected by multiplex immunofluorescence in the lung AC. A and H: CK(cyan); B and K: PD-L1(orange); C and J:CD3(red); D and O:CD68(yellow); E: unmixed composite image for CK, PD-L1, CD3 and CD68 , co-expression of CK and PD-L1(white arrow showed); F: co-expression of PD-L1 and CD68 (white arrow showed); G: co-expression of PD-L1 and CD3 (white arrow showed); I and N: PKM2(green); L: unmixed composite image for CK, PKM2, PD-L1 and CD3, co-expression of CK, PKM2 and PD-L1(white arrow showed); M: co-expression of PKM2 and CD3 (white arrow showed); P: co-expression of PKM2 and CD68 (white arrow showed) (original magnification of all images ×400).