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. 2020 May 18;11(15):4431–4441. doi: 10.7150/jca.42308

Figure 5.

Figure 5

LncHOTAIR/miR-206 targeted CCL2 in CRC cells. A. Bioinformatics analysis of CCL2 and miR-206. B. CCL2 showed high expression in CRC tissues compared to normal tissues by qRT-PCR. C. Flow cytometry assay was performed to show CCL2 knockdown accelerated the apoptosis of SW620 cells. D. The mRNA level of CCL2 was detected in CRC cells transfected with miR-206 mimic by qRT-PCR assay. E. The protein level of CCL2 was measured in CRC cells transfected with miR-206 mimic by western blotting. F. The mRNA level of CCL2 was detected in CRC cells transfected with HOTAIR knockdown by qRT-PCR assay. G. The protein level of CCL2 was measured in CRC cells transfected with HOTAIR knockdown by western blotting. * P<0.05, ** P<0.01, compared with NC group.