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. Author manuscript; available in PMC: 2020 May 28.
Published in final edited form as: J Vis Exp. 2019 Oct 4;(152):10.3791/60384. doi: 10.3791/60384

Figure 3: Endometrial organoids exhibit characteristics of native tissue after 14 days of follicular phase hormone treatment.

Figure 3:

(A) Trichrome staining was done to visualize collagen (blue) and cells (red). Trichrome stain was performed on endometrial tissue (left panel) and endometrial organoids (Scale bars = 20 μm, right panel). (B) Immunohistochemical staining for ER, AR and PR was done in endometrial tissue (Scale bar = 100 μm bottom panels) and endometrial organoids (Scale bar = 20 μm, top panels). Positive stain is shown in brown and hematoxylin stain solution was added as the counter stain shown in blue. Adapted from Teerawat et al., JCEM, (2019)11.