Table of Materials
| Name of Material/Equipment | Company | Catalog Number |
Comments/Description |
|---|---|---|---|
| Collagenase, Type II, powder | Thermo Fisher Scientific | 17101015 | |
| DNase I | Sigma-Aldrich | D4513 | |
| Dispase | Corning | 354235 | |
| Hank's Balanced Salt Solution (HBSS) | Corning | 21-022-CV | 1x without calcium, magnesium, and phenol red |
| MammoCult Human Medium Kit | STEMCELL Technologies | 05620 | supplemented with 2uL/ml heparin and 5 uL/ml hydrocortisone |
| Heparin solution | STEMCELL Technologies | 07980 | added to MammoCult media |
| Hydrocortisone stock solution | STEMCELL Technologies | 07925 | added to MammoCult media |
| Penicillin-Streptomycin | Thermo Fisher Scientific | 15140122 | |
| MicroTissues® 3D Petri Dish® | Sigma-Aldrich | Z764043 | |
| Agarose HS, molecular biology grade | Denville Scientific | CA3510-6 | |
| Ammonium chloride (NH4Cl) | Amresco | 0621 | |
| Sodium bicarbonate (NaHCO3) | Sigma-Aldrich | S6014 | |
| EDTA | Fisher Scientific | BP120-1 | |
| β-Estradiol | Sigma-Aldrich | E2257 | |
| Testosterone | Sigma-Aldrich | 86500 | |
| Phosphate buffered saline, pH 7.4 | Sigma-Aldrich | P3813 | |
| Paraformaldehyde, 16% solution | Electron Microscopy Sciences | 15710 | |
| Direct-zol™ RNA MicroPrep | Zymo Research | R2060 | |
| Hematoxylin Stain Solution | Thermo Fisher Scientific | 3530-32 | Modified Harris formulation, mercury free |
| Eosin Stain | VWR | 95057-848 | |
| Purified mouse anti-E-cadherin antibody | BD Biociences | 610181 | Clone 36 |
| Recombinant anti-vimentin antibody [EPR3776] | Abcam | ab92547 | |
| Trichrome Stain | Abcam | ab150686 | |
| Estrogen Receptor (SP1), rabbit monoclonal antibody | Thermo Fisher Scientific | RM-9101-S | |
| Progesterone Receptor, PgR 1294, unconjugated, culture supernatant | Agilent Technologies | M356801-2 | |
| Fluoroshield with DAPI, histology mounting medium | Sigma-Aldrich | F6057 |