Table 1.
Pnrc2 SH3 domain and NR-box are important for cyclic gene transcript decay
| Condition | her1 expression a | ||
|---|---|---|---|
| Normal b | Accumulated b | Percent affected | |
| Uninjected | 14/29 | 15/29 | 51.7% |
| Cerulean-pnrc2 | 48/49 | ¼9 | 2.0% |
| Cerulean-pnrc2ΔNR (c) | 22/27 | 5/27 | 18.5% |
| Cerulean-pnrc2W->A (d) | 26/50 | 24/50 | 52.0% |
At 16–18 hpf, pnrc2 mRNA-injected embryos from a cross of a pnrc2 homozygous female to a pnrc2 heterozygous male were processed by her1 in situ hybridization. The cross yields 50% Mpnrc2 mutants with normal her1 expression and 50% MZpnrc2 mutants with accumulated her1 expression unless there is phenotypic rescue by the injected pnrc2 mRNA.
Chi-square analysis indicates a significant difference in her1 expression between Cerulean-pnrc2 mRNA-injected and uninjected MZpnrc2 mutant embryos (p < 0.0001) and between Cerulean-pnrc2ΔNR mRNA-injected and uninjected MZpnrc2 mutant embryos (p = 0.001) and no significant difference between Cerulean-pnrc2W->A mRNA-injected and uninjected MZpnrc2 mutant embryos (p = 0.7773).
ΔNR = deletion of NR box
W->A = W124A mutation