Figure 5.

Differential gene expression comparing each major fibroblast cluster (myofibroblast, SPINT2^hi fibroblast, MFAP5^hi fibroblast) to the other two. A-C Volcano plots include all differentially expressed genes with absolute value log2 fold change>0.5, and are colored by adjusted p-value <0.05 or not. D-F Violin plots demonstrate gene expression of selected distinguishing genes for each fibroblast subpopulation. G-I Enriched GO biological processes for the differentially expressed up-regulated and down-regulated genes for each comparison of one major fibroblast cluster to the other two. Functional enrichment analysis was performed using DAVID with all differentially expressed genes with adjusted p-value <0.05 and absolute value log2 fold change>0.5 included. (A), (D), and (G) display results of myofibroblast to SPINT2 ^hi fibroblast and MFAP5 ^hi fibroblast comparison. (B), (E), and (H) display results of SPINT 2 ^hi fibroblast to myofibroblast and MFAP5 ^hi fibroblast comparison. (C), (F), and (I) display results of MFAP5 ^hi fibroblast to myofibroblast and SPINT2 ^hi fibroblast comparison. DAVID, Database for Annotation, Visualization, and Integrated Discovery.