Figure 4.

Can-mediated sensitization of TRAIL-mediated apoptosis is rescued by autophagy attenuation. DU145 cells were pretreated with CQ (10 μM) for 1 h prior to their exposure to Can (1 μM) for 18 h and then to TRAIL for an additional 2 h. (A) The morphology of the cells was evaluated by interference light microscopy (scale bar=100 μm; magnification, ×100). (B) Cellular viability was assessed by crystal violet staining. (C) Bar chart demonstrating the average density of crystal violet staining. (D) Cellular viability was evaluated with trypan blue dye exclusion assays. ^*P<0.05 and ^**P<0.0001 vs. control and each treatment group, respectively. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; CQ, chloroquine; Can, cantharidin.