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. 2020 Apr 6;46(1):280–288. doi: 10.3892/ijmm.2020.4566

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Copyright: © Nazim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Can-enhanced TRAIL-mediated apoptosis is rescued by genetic attenuation of autophagy. DU145 cells were pretreated with ATG5 siRNA or negative control siRNA for 24 h prior to their exposure to Can (1 μM) for 18 h and then to TRAIL for an additional 2 h. (A) The morphology of the cells was evaluated by interference light microscopy (magnification, ×100). (B) Cellular viability was assessed by crystal violet staining. (C) Bar diagram demonstrating the average density of crystal violet staining. (D) Cellular viability was evaluated by trypan blue dye exclusion assays. ^*P<0.05, ^**P<0.01 and ^***P<0.0001 vs. control group and each treatment group, respectively. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; Can, cantharidin; ATG5, autophagy related 5; siRNA, small interfering RNA; NC, negative control.