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. 2020 Apr 14;46(1):97–106. doi: 10.3892/ijmm.2020.4577

Figure 5.

Figure 5

Sevo blocks the PTEN/Akt/GSK-3β/β-catenin signaling pathway by modulating the expression of miR-25-3p in HCC cells. HCCLM3 and Huh7 cells were transfected with miR-25-3p mimics or miR-25-3p inhibitor for 24 h followed by Sevo treatment for 6 h. (A) Protein levels of p-Akt, Akt, p-GSK-3β, GSK-3β, β-catenin, c-Myc and MMP9 were measured using western blotting. β-actin served as the loading control. (B) The bands were semi quantitatively analyzed using ImageJ and normalized to β-actin density. Data are presented as the mean ± standard deviation. (n=3) of three representative experiments. *P<0.01 and **P<0.01 vs. NC group. ##P<0.01 vs. Sevo + mimics NC. PTEN, phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Sevo, sevoflurane; NC, negative control; SD, standard deviation; miR/miRNA, microRNA; MMP, matrix metalloproteinase; PTEN, phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; GSK, glycogen synthase kinase; p-Akt, phosphorylated-protein kinase B.