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The efficiency of knockdown in TRPC1 expression by TRPC1 siRNA was determined by RT-qPCR and western blot analysis. (A) The level of the TRPC1 mRNA was determined by RT-qPCR. (B and C) The level of TRPC1 protein was determined by western blot analysis. Values in (A and C) are shown as the means ± SD (n=3). *P<0.05 vs. the control group.
