Figure 4.

Fluorescence intensity of intracellular Ca²⁺ levels in 16HBE cells was determined using laser confocal microscopy. (A) Representative images of fluorescence-positive cells stretched for 0, 0.5, 1, 2, 4, 8, 12, 24 and 48 h at 15% elongation respectively. (B) The fluorescence intensities for intracellular Ca²⁺ levels in (A) were analyzed with the quantification tools and were shown by fold of control. (C) Representative images of fluorescence-positive cells stretched for 48 h at 15% elongation with or without transfection of TRPC1 siRNA or NC siRNA. (D) The fluorescence intensities for intracellular Ca²⁺ in (C) were analyzed with the quantification tools and are shown as the fold of the control. Values in (B and D) are shown as means ± SD (n=3). ^*P<0.05 vs. the control group.