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. 2020 Apr 7;46(1):320–330. doi: 10.3892/ijmm.2020.4568

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Epithelial-mesenchymal transition (EMT) markers in 16HBE cells after stretching with 15% elongation for 48 h were determined using immunofluorescence, RT-qPCR and western blot analysis. (A) Representative images of fluorescence-positive cells stretched for 48 h at 15% elongation. (B) The fluorescence intensities for EMT markers were analyzed with the quantification tools and were shown by fold of control. (C) The levels of the EMT markers mRNA were determined using real-time RT-qPCR. (D and E) The protein levels of the EMT-related markers were determined by western blot analysis. Values in (B, C and E) are shown as the means ± SD (n=3). ^*P<0.05 vs. the control group. ^#P<0.05 vs. the stretch group.