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. 2020 Apr 22;46(1):159–166. doi: 10.3892/ijmm.2020.4586

Figure 1.

Figure 1

LncRNA BCAR4 is associated with liver cancer progression. (A) The relative expression of lncRNA BCAR4 in liver cancer tissues and normal healthy tissues was measured by RT-qPCR. The expression was normalized to that of 18S RNA. (B) RT-qPCR was performed to determine the expression of lncRNA BCAR4 in non-metastatic (n=18) and metastatic (n=12) liver cancer samples. The expression was normalized to that of 18S RNA. (C) RT-PCR was used to examine the expression of lncRNA BCAR4 in stage I and II (n=14) and stage III and IV (n=16) liver cancer samples. The expression was normalized to that of 18S. (D) Overall survival rate of high and low lncRNA BCAR4 expression groups was analyzed by Kaplan-Meier analysis and the long rank test. All experiments were repeated independently three times. *P<0.05. LncRNA, long non-coding RNA; BCAR4, breast cancer anti-estrogen resistance 4; RT-qPCR, reverse transcription-quantitative PCR.