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. 2020 Apr 8;46(1):211–223. doi: 10.3892/ijmm.2020.4570

Figure 3.

Figure 3

Overexpression of LRP6 enhances migration and invasion of trophoblast cells. Negative control (siNC) and siLRP6 were transfected into JEG-3 cells, whereas HTR8/SVneo cells were transfected with LRP6 or empty pcDNA3.1 vector (Mock), and un-transfected cells served as a control for comparison. (A-F) Microscopic images and quantitative analysis of wound healing and Transwell assays in cells following transfection. (G-L) Western blot analysis and RT-qPCR analyses were performed to determine the expression levels of genes and proteins associated with migration and invasion (i.e., MMP-2, MMP-9, TIMP-1, TIMP-2). The magnification of the images (either x100 or ×200) is indicated in the Figure parts, where applicable.**P<0.001, *P<0.05 vs. siNC or Mock (n=3). ns, not significant; LRP6, low-density-lipoprotein receptor-related protein 6; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metal-loproteinase.