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. 2020 Apr 8;46(1):211–223. doi: 10.3892/ijmm.2020.4570

Figure 4.

Figure 4

LRP6 is the target gene for miR-346. (A) TargetScan predicted that the nt positions 99-105 of the LRP6 3′-UTR could bind to miR-346. miR-346 mimics or blank control were co-transfected into HTR8/SVneo and JEG-3 cells with the constructed carriers containing wild-type or mut LRP6 3′-UTR. (B and C) Double-luciferase reporter analysis was performed to determine the luciferase activity of cells following co-transfection. (D) RT-qPCR was performed to detect miR-346 expression in the placentas of patients with PE and normal pregnant controls. (E) RT-qPCR was performed to detect the level of miR-346 expression in trophoblastic cells. **P<0.001 vs. Control + LRP6-3′-UTR; ##P<0.001 vs. miR-346 mimics + LRP6-3′-UTR mut (n=3). mut, mutant; 3′-UTR, 3′-untranslated region; LRP6, low-density-lipoprotein receptor-related protein 6.