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. 2020 Apr 24;46(1):67–82. doi: 10.3892/ijmm.2020.4589

Figure 8.

Figure 8

Juglanin-induced suppression of ROS generation is dependent on Nrf2 activity via the inactivation of the MAPK signaling pathway. (A) Western blot analysis was used to calculate p38, ERK1/2 and JNK phosphorylation. (B) p-p38, (C) p-ERK1/2, and (D) p-JNK expression levels were calculated following immunoblotting analysis. (E) Nrf2 was silenced using a specific Nrf2 siRNA sequence. Western blotting was performed to calculate Nrf2 expression levels following knockdown. The representative images are presented. (F) The HaCaT cells were pretreated with juglanin administration in the presence or absence of Nrf2 siRNA for 12 h, and exposed to UVB for 24 h, followed by juglanin administration for another 12 h. Immunoblotting analysis was performed to evaluate p-p38, p-ERK1/2 and p-JNK levels for investigating the role of Nrf2 in juglanin-treated cells following UVB exposure. Data are presented as the mean ± standard error of the mean (n=8). **P<0.01 and ***P<0.001 vs. the Con group. +P<0.05, ++P<0.01 and +++P<0.001 vs. the UVB group. ROS, reactive oxygen species; MAPK, mitogen activated protein kinase; Nrf2, nuclear factor-E2-related factor 2; UVB, ultraviolet B; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinases; Con, control.