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. 2020 Apr 24;46(1):67–82. doi: 10.3892/ijmm.2020.4589

Figure 9.

Figure 9

Juglanin reduces the inflammatory response by suppressing NF-κB associated with Nrf2 activation. (A) Inflammatory cytokines, including COX2, IL-1β and TNF-α, were calculated to investigate their gene expression levels using reverse transcription-quantitative polymerase chain reaction analysis. (B) COX2, IL-1β and TNF-α protein expression levels in cells treated under various conditions were assessed via western blotting. (C) The quantification of COX2, IL-1β and TNF-α is presented. (D) Western blotting was performed to evaluate NF-κB phosphorylation in HaCaT cells. (E) The HaCaT cells were pretreated with juglanin in the presence or absence of Nrf2 siRNA for 12 h, and then exposed to UVB for 24 h, followed by juglanin administration for another 12 h. Then, the NF-κB phosphorylation, COX2, IL-1β and TNF-α protein expression levels were calculated by western blot analysis. Data are presented as the mean ± standard error of the mean (n=8). ***P<0.001 vs. the Con group. +P<0.05, ++P<0.01 and +++P<0.001 vs. the UVB group. NF-κB, nuclear factor-κB; Nrf2, nuclear factor-E2-related factor 2; COX2, cyclic oxidase 2; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; p, phosphorylated; NF-κB, nuclear factor-κB; Con, control; UVB, ultraviolet B.