Figure 2.

Two‐dimensional super‐resolution spatial distributions and 3D transport routes of various IDPs with different molecular weights. The molecular weight (i), disordered level (ii), 2D super‐resolution spatial distribution superposed with the 2D physical schematic of NPC in the (X, Y) coordinate plane (iii), and 3D spatial probability density map superposed with the 3D physical schematic of NPC (a cut‐away view) in the (X, R, Ɵ) cylindrical coordinate system for each IDP candidate were provided. In addition, a histogram of spatial probability density for the radial dimension was shown in iv to highlight either a single peak representing a central axial transport route or two peaks representing a peripheral transport route. (a) Nsp1 (1–603) refers an FG segment (AA 1–603) of yeast Nsp1 with a molecular weight of 60 kDa (i). With a high disordered level (ii), Nsp 1 (1–603) was found to mainly diffuse through the NPC's central axial channel, based on its 2D super‐resolution spatial distribution (black dots in iii) and 3D spatial probability density map (red clouds in NPC's axial and radial views shown in iv). N, nucleus; C, cytoplasm. (b–g) Defined similarly as in A, the major information and results for Nup159 (441–881), Nup42 (1–372), Nup116 (348–458), SLD2 (1–453), Coilin (1–576), and CREST (1–402) were presented. IDP, intrinsically disordered protein; NE, nuclear envelope; NPC, nuclear pore complex