Table 2.
Nuclear transport kinetics of protein candidates
| Candidate | IDPs | Dextrans and folded proteins | |||||||
|---|---|---|---|---|---|---|---|---|---|
| SLD2 | CREST | Charged Nup116 (348–458) | WT Nup116 (348–458) | Dextran | Dextran | Dextran | GFP | 2xGFP | |
| MW (kDa) | 52 | 43 | 11 | 11 | 10 | 40 | 70 | 27 | 54 |
| Ch/Hy ratio | 1.08 | 0.30 | 0.74 | 0.09 | ‐ | ‐ | 0.12 | 0.12 | |
| Major spatial transport route | Central | Peripheral | Central | Peripheral | Central | Central | ‐ | Central | ‐ |
| Dwell time (ms) | 1.4 ± 0.5 | 3.0 ± 1.0 | 1.4 ± 0.8 | 2.7 ± 1.2 | 1.2 ± 0.2 | 2.0 ± 0.4 | ‐ | 2.7 ± 1.2 | ‐ |
| Import efficiency (%) | 36 ± 4 | 56 ± 4 | 39 ± 4 | 51 ± 5 | 51 ±4 | 26 ± 4 | 4 ± 2 | 47 ± 5 | 6 ± 6 |
| Export efficiency (%) | 50 ± 4 | 49 ±4 | 39 ± 4 | 50 ± 5 | 51 ±4 | 26 ± 4 | ‐ | 47 ± 5 | ‐ |
Note: In our measurements, more than 200 transport events were collected from 10 different NPCs of 10 cells to determine the transport kinetics for each candidate.
Abbreviations: Ch/Hy, charged/hydrophobic; CREST, calcium‐responsive transactivator; GFP, green fluorescent protein; IDP, intrinsically disordered protein; NPC, nuclear pore complex.